Reporter

Part:BBa_K414004

Designed by: Matthew Polasko   Group: iGEM10_Nevada   (2010-09-28)


Strong Plant RBS (Kozak sequence) + EYFP from E0030

In order for Enhanced Yellow Fluorescent Protein (EYFP)to function properly in plants, a plant-specific ribosome binding site (Kozak sequence)must be added to the 5'region just upstream from the start of translation.The Kozak sequence is AAA AAA AAA ACA and is directly adjacent to the ATG start codon in EYFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None